Formal Report on Exp 5 - 1412 Words

COLUMN AND THIN LAYER CHROMATOGRAPHY Mark Paul P. Pastrana, Mariah Ericka M. Patawaran, Princess Juneire M. Peligro, Francisco Q. Pua III, Rose Anne L. Quyo and Janille P. Ragpa Group 8 2B Medical Technology Organic Chemistry Laboratory ABSTRACT The main objectives were to separate the colored components of malunggay leaves by means of column chromatography, as well as to determine the purity of the components using thin layer chromatography (TLC) and measure the Rf values of the colored components obtained herein. For column chromatography, the sample prepared was loaded into a Pasteur pipette plugged with cotton and uniformly packed with silica gel. The eluents used were 7 mL hexane:acetone (7:3), 5 mL hexane:acetone (1:1), 5 mL†¦show more content†¦Without letting the column run dry, hexane:acetone (1:1) was introduced into the column, and in the same manner the eluates were collected. This was the same for the succeeding eluents, and went on until no more colored eluates could be obtained from the column. Cotton Cotton Silica gel Silica gel Pasteur pipette Pasteur pipette Iron clamp Iron clamp Iron stand Iron stand Figure 2 Column Chromatography 3. Thin Layer Chromatography The eluates obtained from column chromatography were applied on a TLC plate pre-coated with silica by spotting it seven times per color using a capillary tube. Each spot was dried before applying the next. A developing chamber was prepared by placing an amount of the solvent system, hexane:acetone (7:3), into a beaker. Filter paper was used to line the walls of the beaker, and was then covered with a watch glass to equilibrate the chamber. Once the filter paper was saturated with the solvent system, the TLC plate was carefully placed in the beaker to develop. When the solvent system had reached about a centimeter from the upper end of the TLC plate, the plate was removed, and before allowing it to air-dry, the solvent front was marked. Once air-dried, the plate was placed under a UV lamp to visualize the components to determine any additional colors that were invisible without UV light. Solvent system Solvent system Beaker Beaker Watch Glass Watch Glass Figure 3.1 Thin Layer Chromatography Figure 3.2 Thin LayerShow MoreRelatedClass Notes: Intermediate Accounting Chapter 31125 Words   |  5 Pagesout temp accts. 4 kinds of closing entries: 1) close out Revenue - debit Rev CR Income Summ (normal credit bal) (only exists long enough for closing entries) - companies may be closing hundreds of rev or exp accts 2) close out Expense accts - list every single acct DR Income Summ CR Exp accts 3) DR Income Summ CR Retained Earnings 4) DR Retained earnings - return of equity, has nothing to do with production (â€Å"Income† Summ does) CR Dividends (always has deb bal) Ex3-14: 1) DR RevenueRead MoreAccounting for Partnership Business Essay2574 Words   |  11 PagesTable of Contents Introduction 1 Sole trader 1 Partnership 2 Company 2 Registration Process 3 Financial Report 5 Conclusion 10 References 11 Introduction Christina Adams and David Ng recently completed their professional accounting qualifications, registered as tax agents and obtained all the necessary individual licenses as well as professional registrations required to operate as individuals. 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